Validation of Plasmodium falciparum antigens targeted by human CD8+ T cells using T Cell Receptor-expressing reporter cells
Coordenador(es):
- Helder Takashi Imoto Nakaya
Participante(s):
2020 - Atual
Validation of Plasmodium falciparum antigens targeted by human CD8+ T cells using T Cell Receptor-expressing reporter cells
Descrição: In collaboration with Dr Helder Nakaya (Scientific Platform Pasteur USP, Brazil) an expert in systems vaccinology and single-‐cell immunoprofiling25,26, we intend to use Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-‐Seq)29 to single-‐cell sequence CTLs using barcoded dextramers harboring the epitopes discovered by immunopeptidomics. This method will allow us to maximize the use of the small numbers of specific T cells from the humanized mice to retrieve the sequences of epitope-‐specific variable regions of T cell receptors (TCRs). TCRs will be expressed on reporter cells which will become fluorescent after TCR activation by the complex peptide-‐MHC class I molecule30. Thus, to validate Pf ATCs instead of focusing on the elimination of infected hepatocytes, which can depend on non-‐specific factors, such as secreted cytokines in vitro, or yet on the high amounts of CTLs that will be determined by the immunization method/model in vivo, we propose to rely on the specific-‐TCR activation of reporter cells by epitopes presented on the surface of Pf infected hepatocytes..
Situação: Em andamento; Natureza: Pesquisa.
Integrantes: Helder Takashi Imoto Nakaya - Integrante / Rogerio Amino - Coordenador.
Financiador(es): Institut Pasteur - Auxílio financeiro.
Última atualização em 2024-01-15 11:54:00